A. M. Aliyu, S. Duru, G. S. Bawa, S. B. Abdu, M. R. Hassan


This study was carried out to investigate the effect of dietary enzyme (Fullzyme ®) supplementation on haematological parameters of growing rabbits fed boiled sorrel seed meal (BSSM) based diets. A total number of thirty six (36) weaner rabbits of mixed breeds and sexes aged 7-8 weeks (450 – 500 ± 50g) old were randomly allotted into four (4) dietary treatments with three (3) replicates of three rabbits each in a completely randomized design. Four experimental diets were formulated with enzyme supplementation at 0, 0.15, 0.25 and 0.35%, respectively. The experiment lasted for 56 days.  Blood samples were collected from three rabbits randomly selected from each treatment at the end of the study for haematological evaluations. Results revealed no significant differences (P>0.05) in all parameters measured except for neutrophils (P< 0.05). Rabbits fed 0.25% enzyme supplementation had 27% lower values of neutrophils than other treatment groups. It was hence concluded that the higher the number of neutrophils present in the blood, the more the level of infection while the lower the number, the lesser the level of infection or may be absent in the blood.


Boiled Sorrel Seed Meal, Haematological Parameters, Fullzyme, Rabbits

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A.O.A.C. (2005). Official Methods of Analysis, 15th Ed. Association of Official Analytical Chemistry, Washington, DC.

Abdu, S. B., Adegoke, T. F., Abdulrasheed, M., Duru, S., Yashim, S. M. and Jokthan, G. E. (2008). Effect of cooking duration on chemical composition of Roselle Seed. In: Bawa, G. S., Akpa, G. N., Jokthan, G. E., Kabir, M. and Abdu, S. B. (Editions) 13th Proceedings of the Animal Science Association of Nigeria (ASAN) at A.B.U Zaria, pp521 – 523.

Abeke, F. O. (1997). Responses of laying hens to dietary levels of Sheep manure. M.Sc. Thesis, Department of Animal Science, Ahmadu Bello University Zaria.

Abeke, F. O. (2008). Fibre in poultry diet concern and options, in proceeding of the 33rd Annual Conference of the Nigerian Society of Animal Production held at College of Agricultural Science, Olabisi Onabanjo University, Ayetoro, Ogun State, Nigeria. Pp 409-411.

Adanlawo, I. G. and Ajibade, V. A. (2006). Nutritive value of two varieties of rosella (Hibiscus sabdariffa) calyces soaked with wood ash. Pakistan Journal of Nutrition, 5(6): 555-557.

Akanya, H.O., Oyeke, S.B., Jigin, A.A. and Lawal, F.F. (1997). Analysis of Sorrel Drink “Zoborodo”. Nigeria Journal of Biochemistry, 12: 77 – 81.

Akinmutimi, A. H., (2001). Soaked and toasted Lima beans (Phaseolus lunatus) in broiler chickens diets: Effects on haematological parameters and blood chemistry. In: C.O. Ubosi, A. Kibon, I.D. Kwari, J.U. Igwebuike, I.D. Mohammed, N.K. Alade, F.I. Abbator and S.B. Adamu (Eds) 6th Annual Conference of the Animal Science Association of Nigeria, University of Maiduguri, Maiduguri Convocation Square, pp: 48-49.

Ari, M.M., Ayanwale B. A., Adama T.Z. and Olatunji, E. A. (2012). Effect of Different Fermentation Methods on Growth Indices and Serum Profile of Broiler Chickens. Journal of Biology, Agriculture and Healthcare, 2(5):78-86.

Ari, M.M., Ogah, D.M., Hassan, I.D., Musa-Azara, I.S., Yusuf, N.D. and Alu, S.E. (2013). Effect of utilization of crushed, boiled and fermented roselle seeds (Hibiscus sabdariffa L.) on the performance of broiler chickens. British Biotechnology Journal, 4(1): 4-11. www.sciencedomain.org.

Bedford M. R. (2000). Removal of antibiotic growth promoters from poultry diets: implications and strategies to minimise subsequent problems. World’s Poultry Sci. Journal., 56, 347-365.

Chesson, A. and Steward, C. S. (2001). Modulation of the gut microflora by enzyme addition. IN: Piva, A., Bach Knudsen, K. E., Lindberg, J. E. (Editions). Gut Environment of Pigs. Nottingham University Press, Nottingham, 165-179.

Dairo, F. A. S., Adeshinwa, T. A., Oluwasola, T. A. and Oluyemi, J. A. (2011). High and low dietary energy and protein levels for broiler chickens. African Journal of Agricultural Resource, 5(5): 2030 – 2038.

Dashak, D. A. and Nwanegbo, V. (2002). Chemical composition of the seeds of calyxes of Hibiscus sabdariffa grown in Jos North LGA of Plateau. Journal of Natural Sciences, 5: 32 – 34.

Davice, J. U. and Lewis, S. M. (1991). Practical haematology, 8th Edition. Longman Ltd., London, U.K., pp 22-28.

Duru, S. (2010). Effects of different types of rice offal with or without enzyme supplementation on the performance of broiler chickens. A PhD Thesis, Department of Animal science, Ahmadu Bello University, Zaria.

Duwa, H., Oyawoye, E. O. and Njidda, A. A. (2012). Effect of Processing Methods on the Utilization of Sorrel Seed Meal by Broilers. Pakistan Journal of Nutrition, 11(1): 38-46.

Ewuola, E. O., Folayan, O. A., Gbore, F. A., Adebunmi, A. I., Akanji, R. A., Ogunlade, J. T. and Adeneye, J. A. (2004). Physiological response of growing West African Dwarf goats fed groundnut shell-based diets as the concentrate supplements. Journal of Agriculture, 1(1): 61-69.

Fanimo, A.O. Oduguwa, O. O., Alade, A. A., Ogunnaike, T. O. and Adesehinwa, A. K. (2003). Growth Performance, Nutrient Digestibility of Rabbits fed cashew Apple waste. Livestock Research for Rural Development, 15 (8): 112-118.

FAO (Food and Agriculture Organization) (1997). In: The Rabbit: Husbandry, Health and Production. David Lubin Memorial Library cataloguing in publication data, pp. 205.

Frandson, R.D. (1986). Anatomy and Physiology of Farm Animals, 4th Edition. Lea and Febilger. Pennsylvania, USA. pp 240-242.

Gutierrez, I., Espinosa, A., Garcia, J., Carabaño, R. and De Blas, J.C. (2002). Effects of starch and protein sources, heat processing, and exogenous enzymes in starter diets for early weaned rabbits. Journal of Animal Feed Science and Technology, 98:175-186.

Igwebuike, J. U. (2001). Utilization of Acacia products (Acacia albida del) for rabbit feeding. Ph.D Thesis submitted to university of Agriculture. Makurdi, Nigeria.

Ismail, A.E., Ikram, H. K. and Nazri, H.S.M. (2008). Roselle (Hibiscus sabdariffa L.) seeds. Nutritional composition, protein quality and health benefit, Food, 1 – 16.

Joshi, D.C., Katuga, R.C., Adama, T.Z. and Olatunji, E.A. (2012). Effect of different fermentation methods on the proximate composition; amino acid profile, Technology and Bioengineering. Indian Journal of Animal Nutrition, 2: 6 – 13.

Keyembe, N.C. (2011). Germination as a processing technique for soybeans in small scale broiler farming, M. inst. Agrar Dissertation, University of Pretoria, South Africa.

Kwari, I.D., Igwebuike, J.U., Mohammed, I.D. and Diarra, S.S. (2011). Growth, haematology and serum biochemistry of broiler chickens fed raw or differently processed sorrel (Hibiscus sabdariffa) seed meal in a semi – arid environment. International Journal of Science and Nature, 2(1): 22 – 27.

Maikano, M.A., Danlami, M.O., Idris, D.H., Suleiman, M.A., Nuhu, D.T. and Samuel, E.A. (2014). Effects of utilization of crushed, boiled and fermented Roselle seed (Hibiscus sabdariffa) on the performance of broiler chickens. British Biotechnology Journal, 4(1): 21 – 29.

Marquardt, R. R., Brenes, A., Zhang, Z., Boros, D. (1996). Use of enzymes to improve nutrient availability of poultry feedstuffs. Animal Feed Science and Technology. 60: 321 - 330

National Research Council. (1994). Nutrient Requirements of Rabbits. National Academic of Science, Washington, DC, USA.

Neu, H. C., (1992). The crisis in antibiotic resistance. Science 257:1064-1073. http://dx.doi.org/10.1126/science. 25 7.5073.1064 - PMid:1509257.

Nyameh, J., Ribanre, A.J., Alabi, J., Mufwa, B.J. and Gyau, A.M. (2012). Effect of Processing on the Performance and Carcass Yield of Broilers Fed Differently Processed Sorrel (Hibiscus sabdariffa). Journal of Agriculture and Veterinary Sciences, 4: 1-4.

Obadina, A., Oyewole, O., Abiola, S and Sanni, L. (2006). Short communication – fungal enrichment of cassava peels proteins. African Journal of Biotechnology, 5: 302- 304

Ocheja, J. O., Ebiloma, S. O., Ukwuteno, S. O., Oguche, H. G. E., Lalabe, B. C. and Akuboh, C. A. (2011). Performance and Heamatological Parameters of Grower Rabbits Fed With Some Browse Supplemented With A Mixture of Bambara Nut Waste and Rice Offal. Production Agriculture and Technology, 7 (2): 37-47.

Ogundipe, S.O., Saádu, G., Bale, J.O., Aduku, A.O. and Balogun, T.F. (1992). Utilization of animal manure in poultry diets. National Animal Production Research Institute Seminar Series.

Onifade, A. A., Tewe, O. O., Okunola, O. O. and Fanimo, A. O. (1999). Performance of laying pullets fed on cereal diets based on maize offal, Cassava peel and rejected cashew nut meal. British Poultry Science, 6: 61 – 87.

Ovimaps. (2015). Ovi location map; Ovi earth imagery date; July 30th, 2015.

Oyawoye, E.O. and Ogunkunle, M. (1998). Physiological and biochemical effects of raw jack beans on broilers. Proceedings of Annual Conference of Nigerian Society of Animal Production, 23: 141-142.

SAS, (2008). Statistical Analysis System/ State user’s guide, release 9.0 Edition Pub. SAS Institute Inc. SAS Circle, Gary, North Carolina, USA.

Samy, J.C. (1999). Proximate analysis, In: Christie, B.R. (Ed). The Handbook of plant Science in Agriculture, CRC Press, Inc. BocaRaton, Flourida.

Soetan, K.O. and Oyewole, O. E. (2009). The need for adequate processing to reduce the anti – nutritional factors of plants used as human food and animal feeds. A review, African Journal of Food Science, 3(9): 223 – 232.

Steel, R.G.D. and Torrie, J.H. (1998). “Principles and Procedures of Statistics. A Biometrical Approach” 2nd Edition – McGraw Hill Company New York. Pp.481.

Wang, Z. R., Oiao, S. Y., Lu, W. O. and Li, D. F. (2005). Effects of enzyme supplementation in performance, nutrient digestibility, gastrointestinal morphology and volatile fatty acid profiles in the hindgut of broilers fed wheat – based diets. Poultry Science, 84; 875 – 881.


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