EFFECT OF VITAMIN C AND SEMEN EXTENDER ON pH , STORAGE PERIOD AND PERCENTAGE NORMAL CELLS OF CHILLED SEMEN OF FRIESIANXBUNAJI COWS

J N Achi, N P Achi, P P Barje

Abstract


An experiment was conducted to evaluate the effect of vitamin c levels on PH and percentage normal cells of chilled Friesian x Bunaji bull semen in chicken and quail egg yolk extender at different storage periods. The semen was collected from three bulls between 2 – 3 years of age weekly with the aid of artificial vagina. PH was determined using Chemo craft pH paper and pH meter. Extenders were prepared from the egg yolk of chicken and quail at three inclusion levels(0, 3 and 6mg/ml) of Vitamin C. The results shows that the inclusion levels of Vitamin C on chicken and quail egg yolk extender had significant effect (P<0.05) on normal cells of bull spermatozoa but no significant effect (P>0.05) on semen pH at 0, 24, 48 and 72 hours respectively. However, after 72 hours there was significantly higher (P<0.05) normal sperm cells in the quail and chicken egg yolk extenders with higher inclusion level of Vitamin C compared to their control. In conclusion inclusion of Vitamin C up to 6mg/ml in both chicken and quail egg yolk semen extenders maintained semen quality up to 72 hours. 


Keywords


Semen, Friesian x Bunaji, Vitamin C, Semen Extender

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