HISTOPATHOLOGICAL CHANGES ASSOCIATED WITH EXPERIMENTAL TRYPANOSOMA CONGOLENSE INFECTION IN ALBINO RATS

Z. A. Shuaibu, A. I. Lawal, F. U. Samuel, S. O. Ereke, N. A. Sani, M. A. Ibrahim, N. H. Kolo, M. Hussaini, G. O. Fanaiye

Abstract


This work was carried out to evaluate the Histopathological lesions associated with experimental infection of Trypanosoma congolense in albino rats. Ten apparently healthy albino rats were randomly distributed into two groups (A and B). Group A (4 albino rats) which served as control while Group B (6 albino rats) to be infected with T. congolense organism. Each member of group B was inoculated intraperitonially with 1x106 trypanosomes per 0.5 ml of blood obtained from donor mouse and monitored for changes in parasitemia. The peak parasitemia were observed at day 9 PI, the rats were humanely sacrificed and vital organs (brain, lungs heart, liver, spleen, kidney, and intestinal tract) were examined grossly and harvested for histopathological examination using H&E stain. The results revealed grossly pericarditis, hepatitis, nephritis, splenomegaly, petechiae haemorrhages at the intestinal mucosa and histopathological lesions observed were  acute congestion of meningeal capillaries, perivascular Oedema, Neuronecrosis (vaculations), gliosis in the brain, congestion,  Hyperplasia of the alveolar wall, perivascular infiltration of lymphocytes around small blood vessels (venules and arterioles) and thickened inter-alveolar septae infiltrated with mononuclear cells in the lungs,  Vasculitis, mononuclear cellular infiltration,  myocardial necrosis and haemorrhagic myocardium in heart, haemorrhages and acute congestion along with segregation of lymphoid follicles, hyperplasia, reticulo endothelial cells and  considerable amount of amorphous haemosiderin granules in the spleen, Cellular infiltration of the portal triad and vasculitis  in the liver, tubular necrosis, glomerulonephritis and destruction of Bowman’s capsule in the kidney and petechae haemorrhages, necrosis of the intestinal villi, formation of pseudomembrane in the intestinal lumen and depletion of germinal centre of the intestinal tract. It can be concluded that experimental infection of albino rats with Trypanosoma congolense produced significant pathologic changes in vital organs.


Keywords


T. congolense, albino rats, vital organs, gross lesion, histopathological lesions

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References


Adamu, S., Barde, N., Abenga, J.N., Useh, N.M., Ibrahim, N.D.G. and Esievo, K.A.N. (2009). Experimental Trypanosoma brucei infection-induced changes in the serum profiles of lipids and cholesterol and the clinical implications in pigs. Journal of Cell and Animal Biology, 3(2):015-020.

Adewale, A. A., Iyorhemba, U. A., Abah, I. L. and Sani A. (2016). Postpartum pathology in Yankasa ewes experimentally infected with Trypanosoma evansi during pregnancy. Journal of comparative and clinical pathology pp. 593-598.

Allam, L.; Ogwu, D.; Agbede, R. I. S.; Sackey Allam, A. K. B.; Ogwu, L. D.; Agbede, R. I. S. and Sackey, A. K. B. (2011). Hematological and serum biochemical changes in gilts experimentally infected with Trypanosoma brucei. The Journal of Veterinarski Arhiv. 81(5) 597-609.

Auduo, P. A., Esieve, K., Mahammed, G., Ajanusi, O. and Ibrahim, N. (1999). Pathological observations in Trypanosoma evansi infected Yankasa sheep. Journal of Protozoology Research. 9 (2): 64-70.

Bal, M.S., Singla, L.D., Kumar, H., Vasudev, A., Gupta, K. and Juyal, P.D. (2012). Pathological studies on experimental Trypanosoma evansi infection in Swiss albino mice. Journal of Parasitic Diseases. 36 (2): 260-264.

Bisalla, M., Ibrahim, N.D.G., Lawal, I.A. and Esievo, K.A.N. (2007). Serum total protein, albumin and albumin globulin ratio in Yankassa sheep experimentally infected with Trypanosoma congolense and immunomodulated with levamizole. Journal of Protozoology Research, 17:39-43.

Biswas, D., Choudhury, A. and Misra, K. K. (2010). Histopathology of Trypanosoma evansi Infection in Bandicoot Rat. Brain and Choroid Plexus. Zoological Society, Kolakata. 63(1): 27–37.

Dargantes, A.P., Reid, S.A. and Copeman, D.B. (2005). Experimental Trypanosoma evansi infection in the goat. Journal of Comparative Pathology, 133, 267-276.

Davies, J.M. (1997). Molecular mimicry: can epitope mimicry induce autoimmune disease? Immunol. Cell. Biol. 75: 113–126.

Doyle, R.L., Da Silva, A.S., Monteiro, S.G., Santurio, J.M. and Graca, D.L. (2007) Medicines effectiveness for the control of the experimental infection by Trypanosoma evansi in rats. Acta Scientiae Veterinariae. 35, 67-71.

Ekanem, J.T. and Yusuf, O.K. (2008). Some biochemical and haematological effects of black seed (Nigella sativa) oil on T. brucei-infected rats. African Journal of Biomedical Research,11:79-85.

Herbert, W.J. and Lumsden, W.H. (1976). Trypanasoma brucei: A rapid matching method for estimating the host's parasitaemia.Experimental Parasitology, 40:427-31.

Ijagbone, I.F. and Agbede S.A. (2000). A case of congenital transmission of Trypanosoma brucei in mice. Trop Vet, 18:37-38.

Losos, G.J and Ikede, B.O. (1972). Review of pathology of the diseases in domestic and laboratory caused by T. congolense T. brucei, T. rhodesiense and T. Gambiense. Veterinary Pathology. Supplemention ad, 9: 1 - 71.

Mbaya, A.W., Ibrahim, U.I., and Apagu, S.T. (2010). Trypanosomosis of the Dromedary Camel (Camelus dromedarius) and its vectors in the tsetse free zone of North-Eastern Nigeria. Nigerian Veterinary Journal, 31(3): 195 - 200.

Morrison, W.I., Murray, M., Sayer, P.D. and Preston, J.M. (1981). The pathogenesis of experimentally induced Trypanosoma brucei infection in dog. The American Journal of Pathology. 102:182–194.

Nagle, R.B., Dong, S., Guillot, J.M., Mc Daniel, K.M. and Lindsley, H.B. (1980). Pathology of experimental African trypanosomiasis in rabbits infected with T. rhodesiense. The American Journal of Tropical Medicine and Hygiene., 29:1187– 1195.

Ngeranwa, J.J., Gathumbi, P.K., Mutiga, E.R. and Agumbah, G.J. (1993). Pathogenesis of Trypanosoma evansi in small east African goats. Journal of Veterinary Science and Research. 54:283–289.

Nok, A. J., Esievo, K. A. N., Ukoha, I. A., Ikediobi, O. C., Baba, J., Tekdek, Z.,Ndams, I. S. (1992). Kidney Na+- K+- ATP-ase: A kinetic study in rats during chronic infection with Trypanosoma congolense. Journal of Clinical Biochemistry and Nutrition. 13: 72 - 79.

Ogunsanmi, A. O., Akpavie, S. O., Anosa, V. O. (1994). Serum biochemical changes in West Africa dwarf sheep experimentally infected with T. brucei. Revue d’elevage et de Medicine Veterinaire des Pays Tropicaux. 47: 195 - 200.

Onah, D. N.; Hopkins, J. and Luckin, A. G. (1996). Haematological changes in sheep experimentally infected with Trypanosoma evansi. Parasitolgy Research. 82: 629-663.

Pentreath, V.W. and Kennedy, P. G. E. (2004). Pathogenesis of human African trypanosomosis. In: Maudlin, I., Holmes, P.H., Miles, M.A (eds). The Trypanosomiases. CAB International, UK. Pp. 331 - 353.

Prowse, E. (2005). Thesis on Trypanosomiasis, the disease and its control - An analysis of a new tsetse repellant technology. Degree project,46

Samuel, F.U., Adamu, S., Bisalla, M., Chiezey, N.P., Mohammed, A.K., Bello and T.K.(2016). Effect of T.congolense on Haematological Parameters in Experimentally Infected donkeys. Journal of Animal Production Research,28(1):14-24.

Sivajothi, S., Rayulu, V. C., Sujatha, K. and Sudhakara Reddy, B. (2015b). Study of Histopathological Changes in Experimental Trypanosoma evansi Infected Rats. Proceedings of Zoological Society. 68: 112-115

Wang, J., Van Praagh, A., Hamilton, E., Wang, Q., Zou, B., Muranjan, M., Murphy, N.B., Black, S.J., ( 2002). Serum xanthine oxidase: origin, regulation, and contribution to control of trypanosome parasitemia. Antioxid. Redox Signal. 4, 161–178.

World Health Organisation. Tropical Diseases Today: The Challenges and Opportunities WHO, Switzerland, 1975.


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